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| Tabaldi,Luciane Almeri; Ruppenthal,Raquel; Pereira,Luciane Belmonte; Cargnelutti,Denise; Gonçalves,Jamile Fabbrin; Morsch,Vera Maria; Schetinger,Maria Rosa Chitolina. |
| Acid phosphatases (3.1.3.2) are a group of enzymes widely distributed in nature, which catalyze the hydrolysis of a variety of phosphate esters in the pH range of 4-6. We confirmed the presence of acid phosphatases in seedlings of cucumber (Cucumis sativus), radish (Raphanus sativus) and rocket salad (Eruca vesicaria) under different assay conditions using a rapid and simple preparation. The results showed that the optimum pH and temperature used for all species were close to 5.5 and 35°C, respectively. The enzyme was inhibited by molybdate, fluoride, azide, levamisole, orthovanadate, Zn2+ and Cu2+. Suramin had no effect on enzyme activity. The acid phosphatase from cucumber, radish and rocket salad hydrolyzed a wide variety of phosphate esters and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucumis sativus; Raphanus sativus; Eruca vesicaria; Phosphatases. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300009 |
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